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PLATELET FLOW CYTOMETRY ASSAYS
Some assays of platelet function are best achieved with flow cytometry, a method that allows for both enhanced analytical sensitivity and minimal mechanical manipulation during processing. Samples from patients who are profoundly thrombocytopenic can be assayed accurately. Surface markers can be accurately quantified even when platelet counts are low.
HEMEX Laboratories is pleased to announce the availability of the following assays.
Platelet RNA Content (Reticulated Platelets)
Cytometric determination of the platelet RNA content allows for evaluation
of thrombopoietic status in thrombocytopenic states and provides for differential
assessment of marrow suppression vs. peripheral consumption disorders.
In post-chemotherapy and post-transplantation states, reticulated platelets
can be used to monitor recovering megakaryopoiesis / thrombocytopoiesis.
Platelet-Associated Immunoglobulin (PA-IgG)
Platelet-associated IgG has been correlated with ITP and immune-based
platelet destruction. When combined with Reticulated Platelet
data, PA-IgG yields additional diagnostic information over that obtained
from either assay alone.
Platelet Surface Markers
Normal surface markers appear on the platelet surface before activation.
Marker deficiencies associated with impaired platelet aggregation may be
congenital (Glanzmann's thrombasthenia, Bernard-Soulier syndrome) or acquired
(myelodysplasia, leukemia, aplastic anemia, marrow suppression or toxicity).
The markers CD41 (gp IIb/IIIa), CD42a (gpIX), CD42b (gpIb), and CD61 (avb3,
vitronectin receptor) are measured.
Platelet Activation Markers
Several new markers appear on the platelet surface during activation.
Two of these, PAC-1 (activated IIb/IIIa) and CD62P (P-selectin), are becoming
clinically useful as markers of platelet activation. CD31 (PECAM)
and CD63 (a lysosomal antigen) are also available and have clinical applicability.
Note: Sample collection for platelet activation studies should be
performed with a 19 ga. (or larger) needle into a second tube, drawn after
other tubes (or discard first 2 cc drawn separately).
Sample Blue top (citrate) or 7 mL yellow top (ACD) anticoagulated blood;
lavender top (EDTA) is acceptable for reticulated platelets and common
surface markers. Call lab for exact requirements.
Turnaround Time: 23 hours.
All results include laboratory physician review and interpretation.